Genetic linkage and physical mapping of the porcine androgen receptor (AR) gene.

Source and Description of Primers. Primers were designed from published bovine and human androgen receptor (AR) sequences (GenBank accession number Z75315 and M27430, respectively). These primers were used to obtain a porcine sequence (submitted to GenBank: accession number AF079783). A total of 247 bp out of the entire 793-bp PCR product shared 90.3% identity with sections of exons 7 and 8 of the human AR gene. Porcine specific primers (AR-3 and AR-4) were then designed to amplify a 160-bp fragment in intron 7 of the AR gene region from porcine genomic DNA.